Method for the decellularization of intact rat liver
نویسندگان
چکیده
منابع مشابه
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15 صفحه اولA method of obtaining a suspension of intact parenchymal cells from adult rat liver.
The perfusion of liver with either citrate or tetraphenyl boron to remove Ca or K+ or with a solution of high osmolarity and alkaline pH yields plenty of cells but they are all damaged. Perfusion of the liver with hyaluronidase and collagenase followed by incubation of liver slices in the same enzyme solution produced a high yield of cells (25 %, w/w, of liver) of which only about 1 % were unda...
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ژورنال
عنوان ژورنال: Organogenesis
سال: 2010
ISSN: 1547-6278,1555-8592
DOI: 10.4161/org.6.2.11546